Is 25Hz enough to accurately measure a dynamic change in the ocular accommodation?

BACKGROUND Accommodation is often recorded at a low sampling rate using devices such as autorefractors that are designed to measure the static refractive error. It is therefore important to determine if that resolution is sufficient to accurately measure the dynamic properties of accommodation. The current study provides both theoretical and empirical evidence on the ideal sampling rate necessary to measure a dynamic response. METHODS Accommodative and disaccommodative step stimuli ranging from 1-3D (1D steps) were presented using a Badal optical system. Responses from 12 children (8-13 years) and 6 adults (20-35 years) were recorded using a dynamic photorefractor (DPR). Fast Fourier transformation was applied to the unsmoothed dynamic responses including position, velocity and acceleration. Also, velocity and acceleration main sequence (MS) characteristics were compared between three photorefractor conditions on 3 subjects. RESULTS The Nyquist sampling limit necessary to accurately estimate position, velocity and acceleration was at least 5, 10 and 70Hz, respectively. Peak velocity and acceleration were significantly underestimated at a lower rate (p<0.5). However, the slope of MS remained invariant with sampling rate (p>0.5). CONCLUSION Contrary to the previous findings, a dynamic accommodative response exhibited frequencies larger than 10Hz. Stimulus direction and amplitude had no influence on the frequencies present in the dynamic response. Peak velocity and acceleration can be significantly underestimated when sampled at a lower rate. Taken as a whole, low sampling rate instruments can accurately estimate static accommodation, however, caution needs to be exercised when using them for dynamic accommodation.